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fitc labeled hc cath  (MedChemExpress)
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MedChemExpress fitc labeled hc cath
Fitc Labeled Hc Cath, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin tagged cath ka bound streptavidin resin  (Thermo Fisher)
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Thermo Fisher biotin tagged cath ka bound streptavidin resin
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Biotin Tagged Cath Ka Bound Streptavidin Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cath mri  (Philips Healthcare)
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Philips Healthcare cath mri
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Cath Mri, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cathe m  (Biocare Medical)
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Biocare Medical cathe m
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Cathe M, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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port a cath insertion  (Tabuk Pharmaceuticals)
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Tabuk Pharmaceuticals port a cath insertion
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Port A Cath Insertion, supplied by Tabuk Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3f slip cath infusion catheter  (Cook Medical Inc)
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Cook Medical Inc 3f slip cath infusion catheter
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
3f Slip Cath Infusion Catheter, supplied by Cook Medical Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast cath  (Abbott Laboratories)
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Abbott Laboratories fast cath
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Fast Cath, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast cath arterial sheath  (Abbott Laboratories)
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Abbott Laboratories fast cath arterial sheath
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Fast Cath Arterial Sheath, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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allura xper fd20 cath angio system  (Philips Healthcare)
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Philips Healthcare allura xper fd20 cath angio system
Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured <t>with</t> <t>biotin-tagged</t> <t>Cath-Ka-bound</t> <t>Streptavidin</t> resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant
Allura Xper Fd20 Cath Angio System, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured with biotin-tagged Cath-Ka-bound Streptavidin resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Cathelicidin-Ka, the first frog-derived TLR2 and TLR4 agonist, induces macrophage activation and promotes inflammation

doi: 10.1007/s00018-025-06068-y

Figure Lengend Snippet: Cath-Ka directly binds to both TLR2 and TLR4. ( A ) Flow cytometry measurement of the binding reaction of FITC-labeled Cath-Ka (1.25–20 µM) with RAW264.7 cells. ( B ) The effects of TLR inhibitors on Cath-Ka-induced TNF-α production. RAW264.7 cells were pre-treated with the indicated inhibitors for 30 min and then co-cultured with Cath-Ka (10 µM) for 24 h before TNF-α production was measured by ELISA. ( C ) The effects of Cath-Ka (10 µM) on LTA (10 µg/mL) - or LPS (100 ng/mL)- induced TNF-α production in RAW264.7 cells. ( D ) Representative SPRi sensograms of Cath-Ka binding to TLR2 or TLR4 immobilized on a gold chip. Recombinant human TLR2 and TLR4 (1 mg/mL) were immobilized on an activated bare gold SPRi chip before Cath-Ka (0.25–4 µM) was used as the mobile phase. ( E ) Representative WB images of TLR2 protein pulled down by Cath-Ka. TLR2 protein of RAW264.7 cell lysates was captured with biotin-tagged Cath-Ka-bound Streptavidin resin before it was eluted and used for WB analyses. Cath-Ka-1 was used as the negative control. ( F ) Representative WB images of CETSA. RAW264.7 cells were stimulated with Cath-Ka (10 µM) or PBS for 1 h under the indicated temperatures before TLR2 (a) and TLR4 (b) protein expressions were detected by WB. Data are expressed as mean ± SD ( n = 3). *** P < 0.001 is thought statistically significant to the control group, while # P < 0.05, ## P < 0.01, and ### P < 0.001 are deemed to have significant differences to the corresponding Cath-Ka/LTA/LPS treatment groups; ns means not significant

Article Snippet: In brief, RAW264.7 cells (1 × 10 7 cells) at logarithmic growth period were lysed with Pierce IP lysis buffer from Thermo Fisher Scientific (Waltham, MA, USA) and incubated with biotin-tagged Cath-Ka-bound Streptavidin resin overnight at 4°C according to the manufacturer’s instructions (EZ-Link Desthiobiotinylation and Pull-Down Kit, Thermo Fisher Scientific Company).

Techniques: Flow Cytometry, Binding Assay, Labeling, Cell Culture, Enzyme-linked Immunosorbent Assay, Recombinant, Negative Control, Control